ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (9): 1387-1392.doi: 10.11843/j.issn.0366-6964.2014.09.001

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Application of CRISPR/Cas9 System for Gene Regulation

WEI Ze-hui,JIA Cun-ling,ZHANG Zhi-ying*   

  1. (Northwest A & F University,Yangling 712100,China)
  • Received:2014-03-18 Online:2014-09-23 Published:2014-09-23

Abstract:

CRISPR/Cas9 system is one of genome editing technologies developed in 2012.Compared with TALENs and ZFN technologies,CRISPR/Cas9 system has similar gene editing efficiency but is easily manipulated.The engineered dCas9 protein without endonuclease catalytic activity can be obtained by mutating D10A and H840A sites of Cas9 protein.The dCas9 guided by gRNA can be targeted to specific DNA site for the purposes of either inhibiting or activating gene transcription.This paper reviewed the application of CRISPR/Cas9 system in gene regulation as regarding the principle and affecting factors and so on,in order to provide a reference for related research.

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